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  7. Gibco™ Expi293™ Expression Medium

Gibco™ Expi293™ Expression Medium
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Product Code. 13469756
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Quantity:
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6 x 1 L
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1 L
10 L
20 L
6 L
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To receive the discount customers must purchase three of the same product at list price in a single order to receive 33.33% discount. There is no limit to the multiples of 3 that customers can buy. Use promo code ”24112” to get your promotional price
This item is not returnable. View return policy
To receive the discount customers must purchase three of the same product at list price in a single order to receive 33.33% discount. There is no limit to the multiples of 3 that customers can buy. Use promo code ”24112” to get your promotional price

Description

Expi293™ Expression Medium designed for growth, transfection of suspension-adapted human embryonic kidney (HEK) 293 cells.

Expi293™ Expression Medium is a chemically defined, serum-free, protein-free medium for growth and transfection of suspension-adapted human embryonic kidney (HEK) 293 cells. It is specifically designed as a core component to support high density culture of Expi293F™ cells in the Expi293™ Expression System for scalable transient protein expression.

  • Supports growth of suspension 293 cultures to densities of over 1.5 x 10^7 cells/mL
  • Transfection compatible serum-free suspension culture medium enables transfection efficiencies of greater than 70% using ExpiFectamine™ transfection reagent
  • Enables sustained, high level expression of high density transiently transfected cultures, achieving yields of up to 1 gram per liter of recombinant protein
  • Supports growth and transfection of 293 cells in culture formats of less than 1 mL in multiwell plates to greater than 10 L in disposable bioreactors
  • Does not contain phenol red

Expi293™ Expression Medium is formulated with GlutaMAX™-I reagent. It is ready to use, with no supplementation required. The chemically defined formulation results in high reproducibility and lot-to-lot reliability. Expi293™ Expression Medium contains no human or animal-origin components. The medium is not recommended for adherent cell cultures.

TRUSTED_SUSTAINABILITY

Specifications

Cell Line 293E (HEK), 293 (HEK), Expi293F™, FreeStyle™ 293-F Cells, 293F
Cell Type Embryonic Kidney Cells
Classification Animal Origin-free, Chemically-defined, Protein-free, Serum-free
Concentration 1 X
Form Liquid
Product Type Expression Medium
Serum Level Serum-free
Sterility Sterile
Sterilization Method Sterile-filtered
With Additives High Glucose, GlutaMAX, Sodium Pyruvate, Sodium Bicarbonate, Dextrose, Pyridoxine HCl
Without Additives No HEPES, No Phenol Red, No Hypoxanthine, No Thymidine, No Insulin, No Vitamin A, No 2-Mercaptoethanol
Product Line Expi293™
Quantity 1 L
Shipping Condition Room Temperature
Endotoxin Level < 1 EU/mL
Format Bottle(s)
Included Antibiotics No antibiotics
Inorganic Salts Calcium, Magnesium, Sodium Phosphate
Osmolality 275-295 mOsm/kg
Purity or Quality Grade Cell Culture Grade
Content And Storage Store in the dark at +2°C to +8°C
Culture Environment CO2
Culture Type Suspension Cell Culture
Expression System Expi293F™
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Safety and Handling

compliance-icons
Product Identifier
  • Expi293 Expression Medium
 Signal Word
  • Warning
 Hazard Category
  • Serious eye damage/eye irritation Category 2
  • Skin corrosion/irritation Category 2
  • Specific target organ toxicity Category 3
 Hazard Statement
  • H315-Causes skin irritation.
  • H319-Causes serious eye irritation.
  • H335-May cause respiratory irritation.
 Precautionary Statement
  • P261-Avoid breathing dust/fume/gas/mist/vapours/spray.
  • P280-Wear protective gloves/protective clothing/eye protection/face protection.
  • P302+P352-IF ON SKIN: Wash with plenty of water/soap.
  • P305+P351+P338-IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
 Supplemental information
  • MIXTURE LIST-Contains : 4-Isopropoxybenzoicacid

For Research use or Further Manufacturing. Not for diagnostic use or direct administration into humans or animals.

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