Invitrogen™ Human Endostatin ELISA Kit

Beschreibung

The Human Endostatin ELISA quantitates Hu Endostatin in human serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu Endostatin. Principle of the method The Human Endostatin solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

COL18A1 ist die Alpha-Kette von Typ-XVIII-Kollagen. Dieses Kollagen ist eines der multiplexinen, extrazellulären Matrixproteine, die mehrere Triple-Helix-Domänen (kollagene Domänen) enthalten, die von nicht-kollagenen Domänen unterbrochen werden. Das proteolytisch produzierte C-terminale Fragment des Typs-XVIII-Kollagen ist Endostatin, ein potentes antiangiogenes Protein. Mutationen in diesem Gen sind mit dem Knobloch-Syndrom assoziiert. Die Hauptmerkmale dieses Syndroms sind Anomalien der Retina, sodass Typ-XVIII-Kollagen eine wichtige Rolle bei der Netzhautstruktur und beim Verschluss der Neuralröhrchen spielen kann.