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  8. 10734227

Applied Biosystems™ Power SYBR™ Green RNA-to-CT™ 1-Step Kit

The novel formulation reduces false positive results caused by primer dimers, significantly improves data accuracy, and provides reliable detection of low abundance targets.

Marca:  Applied Biosystems™ 4391178

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Codice prodotto. 10734227

  • 432.00CHF / Pezzo
Solo null disponibili
Spedizione stimata: 24-06-2025
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Descrizione

Descrizione

The novel formulation reduces false positive results caused by primer dimers, significantly improves data accuracy, and provides reliable detection of low abundance targets.

  • Get sensitivity and specificity in an easy-to-use one-step qRT-PCR reaction.
  • Formulated to ensure maximum sensitivity and reliability
  • Reduces false positive results caused by primer dimers, significantly improves data accuracy, and provides reliable detection of low abundance targets

Includes:

  • AmpliTaq™ Gold DNA Polymerase UP for hot start qPCR, minimizing non-specific product formation
  • ArrayScript™ UP Reverse Transcriptase, an engineered RT that produces high yields of full-length cDNA
  • An additive that reduces primer dimer formation, reducing non-specific products that can cause false positive signals
  • RNase inhibitor, to prevent degradation of RNA templates
  • A proprietary ROX™ dye that serves as a passive internal reference to normalize non-PCR-related fluorescence fluctuations for superb precision on Applied Biosystems™ real-time PCR instruments
TRUSTED_SUSTAINABILITY
Specifica

Specifica

Sufficient for 40 reactions at 50 μL reaction volume and includes:
• 1 x 1 mL tube of 2X master mix containing SYBR™Green 1 Dye, AmpliTaq Gold™ DNA Polymerase UP, dNTPs, Passive Reference 1, and optimized buffer components. Store at 2-8°C after opening.
• 1 x 20 μL tube of 125X RT enzyme mix containing ArrayScript™ UP Reverse Transcriptase and RNase Inhibitor. Store at -15 to -30°C.


Guaranteed minimum shelf life is 60 days (exact expiry date printed on product and CofA).
Tube
AmpliTaq Gold DNA Polymerase
7500 Fast System, 7500 System, 7900HT System, QuantStudio™ 12k Flex, QuantStudio™ 3, QuantStudio™ 5, QuantStudio™ 6 Flex, QuantStudio™ 7, StepOne™, StepOnePlus™, ViiA™ 7 System
SYBR Green
RNA-to-CT™, SYBR™
UP (Ultra Pure)
ArrayScript™ UP
Wet Ice
Gene Expression
40 Reactions
1 x 1 mL
SYBR
1-step RT-qPCR
Standard
Green RNA-to-CT 1-Step Kit
ROX (Pre-mixed)
Real Time PCR SYBR Master Mix
40 reactions
Standard
40 Reactions at 50 μL
2X (MasterMix), 125X (RT Enzyme Mix)
RNA
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