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  5. IBA Lifesciences pCSG-IBA Mammalian Expression Vector with Secretion

IBA Lifesciences pCSG-IBA Mammalian Expression Vector with Secretion
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Product Code. p-7139101 Shop All IBA Lifesciences Products
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Vector:
pCSG-IBA102
pCSG-IBA104
pCSG-IBA142
pCSG-IBA144
pCSG-IBAwt2
Protein Tag:
His Tag (6x), Twin-Strep-tag
Twin-Strep-tag
Twin-Strep-tag, His Tag (6x)
Untagged
Unit Size:
5µg
This item is not returnable. View return policy
This item is not returnable. View return policy

Description

Designed for high-level episomal expression in mammalian hosts under G418 selection. Mammalian Expression Vector with Secretion is a large expression vector with universal features for transient expression as well as for generation of stable cell lines (NeoR).

pCSG-IBA mammalian expression vectors
  • Designed for high-level episomal expression in mammalian hosts under G418 selection containing the following elements:
  • Human cytomegalovirus (CMV) immediate-early promoter for high-level expression in a wide range of mammalian cells
  • Episomal replication through Epstein Barr Virus replication origin (oriP) and nuclear antigen encoded by EBNA-1 in human, primate and canine cells and through SV40 replication origin in cells latently infected with SV40 or that express the SV40 large T antigen (e.g. HEK293T, COS-1, COS-7)
  • Stable cell lines can be selected by the neomycin resistance gene (NeoR).
  • In addition, the human cytomegalovirus (CMV) immediate-early promoter enables a high-level expression in a wide range of mammalian cells.

Please note that cloning into pCSG-IBA Acceptor Vectors compulsorily requires the restriction enzyme Esp3I since no other MCS for the integration of a gene of interest is available. Besides to the direct cloning of the gene of interest into pCSG-IBA vectors with Esp3I, another option via a so-called entry-vector is possible.

Specifications

Constitutive or Inducible System Constitutive
Marker Gene Ampicillin
Promoter CMV
Product Type Vector
Selection Agent (Eukaryotic) Geneticin™ (G-418)
Content And Storage Recommended: 2°C to 8°C for frequent usage, -20°C for long-term storage
Protein Tag Untagged
Quantity 5 μg
Vector pCSG-IBAwt2
For Use With (Application) Episomal expression (oriP, EBNA-1); BM40 secretion signal
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