Invitrogen™ Mouse IL-20 ELISA Kit

Beschreibung

The Mouse Interleukin-20 (Ms IL-20) ELISA quantitates Ms IL-20 in mouse serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Ms IL-20. Principle of the method The Mouse IL-20 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

Zytokine, die durch Klasse-II-Rezeptoren signalisieren, bilden eine eigene Familie, die die Interferone und Interleukin10 (IL-10) umfasst. Die kürzliche Identifizierung mehrerer IL-10-Homologe hat eine Zytokin-Unterfamilie geschaffen, zu der AK155, IL-19, IL-20, IL-22 und IL-24 gehören. IL-20 gehört zur IL-10-Familie und spielt eine Rolle bei Hautentzündungen und der Entwicklung hämatopoetischer Zellen. Über seine weiteren biologischen Funktionen und klinischen Auswirkungen ist wenig bekannt. Diese Zytokine zeigen pro-entzündliche Aktivitäten in verschiedenen Geweben, einschließlich der Haut.