Thermo Scientific™ PathoDX™ Strep F Grouping Latex

Descrizione

Use Thermo Scientific™ Remel™ PathoDx™ Strep F Grouping Latex with Strep Grouping Kits as replacement reagents. The PathoDx procedure utilizes a latex agglutination method in conjunction with a nitrous acid extraction procedure^1,2. The IgG coupled to the latex is highly specific for a given streptococcal group antigen. This method offers significant advantages over other streptococcal grouping procedures in terms of rapidity, simplicity, and convenience.

The streptococcal group carbohydrates of streptococcal group F is a complex antigen with a rhamnose oligosaccharide and differing side chains, consisting primarily of glucosamine, either acetylated or non-acetylated. In the PathoDx procedure, specific antibody on latex particles reacts with, and agglutinates, streptococcal group antigen extracted from the bacterial cell wall. The test is intended for use with streptococcal colonies that are betahemolytic on sheep blood agar or for use with pure streptococcal isolates in broth cultures. The reagents are designed to give a positive (2+ or greater) agglutination with one to two 18 to 24 hour colonies for most beta-hemolytic streptococcal isolates. Minute colonies of Group F and small colony variants of other streptococci may require 10 colonies or more.

• Ready-to-use – Prepared reagents
• Easy-to-store – Store at 2-8°C
• Easy to interpret – Minute colony Group F and small colony strains of other groups require many more colonies (heavy sweep) to give a positive agglutination reaction. In a negative test, a uniform milky appearance with no agglutination after 60 seconds.

Strep F Grouping Latex (Blue Cap) contains 3.0mL of a suspension of synthetic blue latex particles coated with rabbit antibody IgG-sensitized with 0.098% sodium azide and 0.05% ProClin 300^® as preservatives.

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Remel™ and Oxoid™ products are now part of the Thermo Scientific brand.