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  5. Invitrogen™ RNAqueous™ Total RNA Isolation Kit

Invitrogen™ RNAqueous™ Total RNA Isolation Kit

The RNAqueous Total RNA Isolation Kit is for phenol-free total RNA isolation from 1–75 mg of tissue or from 10^2–10^7 cells, using a guanidinium-based lysis/denaturant and glass fiber filter separation technology.

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Specifications

For Use With (Application) RT-PCR, qPCR, cDNA Library Construction, NGS, Microarray Analysis, Blot Hybridization, Northern Blotting, In Vitro Translation, Nuclease Protection Assays, Nucleic Acid Labeling
Shipping Condition Room Temperature
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Description

Description

The RNAqueous Total RNA Isolation Kit is for phenol-free total RNA isolation from 1–75 mg of tissue or from 102–107 cells, using a guanidinium-based lysis/denaturant and glass fiber filter separation technology.

The RNAqueous kit provides:

  • Convenient syringe-based filtration
  • Compatibility with vacuum manifolds when processing multiple samples

How it works

The RNAqueous kit uses a powerful chaotropic solution for cell lysis, followed by RNA binding to glass fiber housed in a luer-lock syringe filter. Washing and elution solutions are simply pushed through the filter using a standard syringe (not included). Vacuum filtration is not required; however, the filter units are compatible with standard vacuum manifolds for processing several samples at once.

Specifications

Specifications

RT-PCR, qPCR, cDNA Library Construction, NGS, Microarray Analysis, Blot Hybridization, Northern Blotting, In Vitro Translation, Nuclease Protection Assays, Nucleic Acid Labeling
Room Temperature
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Safety and Handling

Safety and Handling

Product Identifier
  • RNAqueous Total RNA Isolation Kit

Signal Word
  • Danger

Hazard Category
  • Acute toxicity Category 4
  • LONG-TERM AQUATIC HAZARD Chronic 3
  • Carcinogenicity Category 1B
  • Serious eye damage/eye irritation Category 2
  • Germ cell mutagenicity Category 2
  • Reproductive toxicity Category 1B
  • Skin corrosion/irritation Category 1 B
  • Skin corrosion/irritation Category 2
  • Skin sensitiser Category 1
  • Specific target organ toxicity Category 2
  • Specific target organ toxicity Category 3

Hazard Statement
  • H302-Harmful if swallowed.
  • H314-Causes severe skin burns and eye damage.
  • H315-Causes skin irritation.
  • H317-May cause an allergic skin reaction.
  • H319-Causes serious eye irritation.
  • H335-May cause respiratory irritation.
  • H341-Suspected of causing genetic defects.
  • H350-May cause cancer.
  • H360-May damage fertility or the unborn child.
  • H371-May cause damage to organs.
  • H412-Harmful to aquatic life with long lasting effects.

Precautionary Statement
  • P201-Obtain special instructions before use.
  • P260-Do not breathe dust/fume/gas/mist/vapours/spray.
  • P261-Avoid breathing dust/fume/gas/mist/vapours/spray.
  • P264-Wash hands thoroughly after handling.
  • P270-Do not eat, drink or smoke when using this product.
  • P273-Avoid release to the environment.
  • P280-Wear protective gloves/protective clothing/eye protection/face protection.
  • P301+P310-IF SWALLOWED: Immediately call a POISON CENTER/doctor /
  • P302+P352-IF ON SKIN: Wash with plenty of water/soap.
  • P303+P361+P353-IF ON SKIN (or hair): Take off immediately all contaminated clothing. Rinse skin with water/ shower.
  • P304+P340-IF INHALED: Remove person to fresh air and keep comfortable for breathing.
  • P305+P351+P338-IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
  • P308+P313-IF exposed or concerned: Get medical advice/attention.
  • P330-Rinse mouth.
  • P332+P313-If skin irritation occurs: Get medical advice/attention.
  • P403+P233-Store in a well-ventilated place. Keep container tightly closed.
  • P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.

Supplemental information
  • MIXTURE LIST-Contains : Lithium chloride, Formamide, Formaldehyde, Methanol, 2-Mercaptoethanol

SDS
Documents

Documents

Product Certifications

For Research Use Only. Not for use in diagnostic procedures.

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